In some of the Windows Server 2008 and 2008 R2 servers which were not patched correctly or not up to date in terms of windows update, you might have some problems accessing BizTalk360 web application when the site is launched. Typically you'll see exceptions saying "No Default Document", "Static Content" etc
The main reason is because correct handler mappings or not configured in IIS 70/7.5. There is a hot fix available from Microsoft to resolve this issue. You can obtain this from this link http://support.microsoft.com/kb/980368.
You may still experience some issues, typically the Windows Communication Foundation extension .svc is not registered correctly. You can rectify this by running the following command
Open Command Prompt as Administrator and navigate to the following folder
and, execute the following command
You may still experience issue with exception something like this
This typically happens when the sequence of steps not performed in order, example ASP.NET was configured first, then WCF registrations happened etc. You can solve this problem by re-registering ASP.NET. You can run the the following command.